PROTEIN PROFILING OF Bryophyllum Pinnatum (LAM.) KURZ. LEAF
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چکیده
Objective: Bryophyllum pinnatum (Lam.) Kurz. is a medicinal herb commonly used to treat ulcers, cough, diabetes and cancer. In this study, phosphate extraction buffer (pH) was used to extract proteins from the leaves of Bryophyllum pinnatum. Method: On sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation, the results showed that most of the extracted proteins migrated in the range of 10 kDa to 30 kDa MW. Bands on the gel was then excised and digested with trypsin and subjected to liquid chromatography tandem mass spectrometry (LC/MS/MS) for protein identification. Result: Proteinase K has been identified from the MS/MS data. The protein identified was Proteinase K, which is used commercially in digesting of unwanted proteins liked keratin.
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